Rapifluor-ms N-glycan Kit User Manual

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The Agilent AdvanceBio Gly-X (formerly ProZyme) technology is a new generation of N-glycan sample preparation. Be ready for sample analysis faster with rapid (5-minute) enzymatic release of N-glycans and labeling with InstantDyes including the brightest in class InstantPC, or traditional reductive amination dyes. Innovative in-solution workflows combined with plate-based rapid cleanup enables sample prep in as little as 1 hour. Increase analytical sensitivity with a variety of dye options, while increasing sample throughput.
  1. Rapifluor-ms N-glycan Kit User Manual Free
  2. Rapifluor-ms N-glycan Kit User Manual Download

GlycoWorks RapiFluor-MS N-Glycan Kit Reimagine Released N-Glycan Analysis. Waters has developed the GlycoWorks RapiFluor-MS N-Glycan Kit.This technology enables unprecedented fluorescent and mass spectrometric performance for glycan detection while also improving the throughput of N-glycan sample preparation. Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in 30 minutes, the GlycoWorks™ RapiFluor-MS™ N-Glycan Kit reduces complicated, time-consuming sample preparation.

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Agilent Gly-Q Glycan Analysis System User Manual 11 Sample preparation and Analysis kits Agilent Gly-X N-Glycan rapid release and Labeling kits (formerly ProZyme) Gly-X sample preparation kits use a novel in-solution enzymatic deglycosylation followed by labeling of released N-glycans with proprietary Ag ilent Instant dyes (formerly ProZyme). Manual guide best laptops 24 tv episode guide book guide note study. Maintenance and Waters 2795 manual. Msi k9n2 sli platinum. GlycoWorks RapiFluor-MS N-Glycan Kit Care and Use Manual The attached user manual covers the Waters e2795 Separations Module. (ManUal Bar staCker. 20x6” travel With video feed, With #zq200 lC/Ms detector s/n- laa262.

Rapifluor-ms N-glycan Kit User Manual Free

N-glycan

Rapifluor-ms N-glycan Kit User Manual Download

  • Four dyes are currently available for the Gly-X platform:
  • InstantPC: a novel instant N-glycan label for liquid chromatography separations that provides markedly increased MS and HILIC-FLR sensitivity. Sample prep time: ~1 hour.
  • 2-AB Express: 2-AB (2-aminobenzamide) has been used to generate labeled N-glycan data for over 20 years and is well established in many laboratories. Traditionally, labeling with 2-AB requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X 2-AB Express on-matrix labeling. Sample prep time: ~2 hours.
  • InstantAB: ProZyme’s original InstantDye which allows room temperature, instant labeling of released N-glycans. Sample prep time: ~1 hour.
  • APTS Express: APTS (8-aminopyrene-1,3,6-trisulfonic acid) is a negatively charged N-glycan label commonly used for capillary electrophoresis (CE) separations. Traditionally, labeling with APTS requires the sample to be dried down prior to the labeling reaction, a step that has been eliminated with Gly-X APTS Express on-matrix labeling. Sample prep time: ~2.5 hours.
  • InstantQ: a negatively charged N-glycan label for capillary electrophoresis (CE), designed for use with our Gly-Q N-glycan analysis platform. Sample prep time: ~1 hour.